目的探讨抗心磷脂抗体(anticardiolipin,ACA)与儿童过敏性紫癜(Henoch-sch¨onlein purpura,HSP)的关系。方法采用酶联免疫吸附试验(ELISA)对91例急性期HSP患儿(HSP组)和30例健康体检儿童(对照组)的血清进行IgG型ACA(IgG-ACA)和IgM型ACA(IgM-ACA)检测,并对两组ACA阳性率进行统计学比较。结果 91例HSP患儿中有16例ACA阳性,其中IgM-ACA阳性13例,IgG-ACA阳性3例,总阳性率占17.6%,且均无抗磷脂综合征(APS)表现;而健康体检儿未检出ACA阳性者。经Fisher精确概率检验,差异有显著性(χ2=6.078,P0.05)。HSP组中50例伴有腹痛的患儿中其13例ACA阳性,阳性率为26.0%;而41例不伴腹痛的患儿中仅3例ACA阳性,阳性率为7.3%,经χ2检验,差异有显著性(χ2=5.426,P0.05)。26例肾脏受损者中ACA阳性4例;65例无肾脏受损者中ACA阳性12例,经χ2检验,差异无显著性(χ2=0.002,P0.05)。结论 ACA可能与儿童HSP发病及腹痛症状有关、与HSP肾损害无关;APS可能与儿童HSP无关。

Objective To discuss the relation between anti-cardiolipin antibodies (ACA ) and Henoch-Scho¨nlein purpura(HSP)in children.Methods The levels of serum IgG-ACA and IgM-ACA in 91 children with Henoch Scho¨nlein purpura in acute stage (HSP group)and 30 healthy cases (control group) were tested with enzyme-linked immunosorbent assay (ELISA ). Statistical analysis were performed to compare the differences of serum ACA levels between two groups.Results 16 patients in HSP group were positive of serum levels of ACA,with 13 of IgM-ACA and 3 of IgG-ACA respectively, and no one manifested signs of antiphospholipid syndrome.None of healthy cases in control group were positive of ACA,the overall positive rate of ACA in HSP group was 17.6% and statistically different (Fisher exact probability test,χ2 =6.078,P 0.05).Conclusions Serum ACA may have a connection with pathogenesis of childhood HSP and abdominal pain,but not correlate with renal damage.Antiphospholipid syndrome probably has n

目的：对新发现的一种新型淀粉蔗糖酶AcAS的结构功能进行深入讨论。方法：用同源模建方法构建AcAS的三维结构；用高斯网络模型和各项异性网络模型，对其功能型运动和工作机理进行预测；利用迭代高斯网络模型方法对其去折叠路径进行预测；根据去折叠路径预测及折叠自由能计算结果设计突变体。结果：模建结果表明，AcAS结构与淀粉蔗糖酶NpAS的结构更相似；AcAS有扭转运动的趋势，其中AcAS的N/C结构域运动性较强，而催化核心的运动性较弱；根据去折叠路径预测，发现N、B和C结构域较易去折叠；通过自由能计算，针对上述3个结构域设计了5株突变体。结论：构建了AcAS的三维结构模型并根据模型探讨了其工作机理；根据去折叠路径预测及折叠自由能计算结果，对AcAS的稳定性改造提出了有益的建议。

Objective: The structure and functions of the newly identified Arthrobacter chlorophenolicus amylosu?crase(AcAS) was thoroughly investigated. Methods: The structural model of AcAS was predicted by homology mod?eling; Gaussian network model and anisotropic network model were used to predict its functional motions and the working mechanism; the unfolding pathway of AcAS was predicted by using the Iterative Gaussian network model;mutants were designed for better stability according to results of unfolding pathway and folding free energy predic?tions. Results: The result of the modeling shows that the structure of AcAS is more similar with that of NpAS;AcAS shows intrinsic twisting motions, wherein the motions of N/C-domains are stronger, while the movement of the catalytic core is weak; unfolding pathway prediction shows N, B, and C domains of AcAS are easier to fold than the other domains; on the basis of free energy calculation, five mutants were designed against the above three domains

探讨复发性流产(RSA)患者甲状腺抗过氧化物酶抗体(TPOAb)、抗心磷脂抗体(ACA)对受孕能力、早期妊娠结局的影响。方法:以2010年7月至2013年2月非妊娠RSA患者183例为研究对象,根据首诊时检测的ACA和TPOAb阳性情况分为ACA阳性组(组I,n=75)、ACA和TPOAb均阳性组(组Ⅱ,n=27)、TPOAb阳性组(组Ⅲ,n=81),比较1年内三组受孕能力及早期妊娠结局。结果:组l的自然妊娠率和早期妊娠成功率均显著高于组Ⅱ和组Ⅲ(P均0.001)。结论:在ACA阳性RSA患者中,常并存TPOAb阳性,且与受孕能力下降、自然流产存在明显关系,建议对RSA患者常规行甲状腺自身抗体检测。

To explore the effect of thyroid peroxidase antibody(TPOAb)and anticardiolipin antibodies (ACA)on ability to conceive and early pregnancy outcome in patients with recurrent spontaneous abortion (RSA). Methods:183 non-pregnant women affected with RSA from Jul 2010 to Feb 2013 were divided into ACA positive group(group I,n=75),both TPOAb and ACA positive group(group Ⅱ,n=27),TPOAb positive group(group Ⅲ,n=81) according to the positive of ACA and TPOAb detected at first diagnose,and the ability to conceive and early pregnancy outcome were compared among the three groups. Results:The rates of natural pregnancy and early pregnancy success in group I were significantly higher than those in group Ⅱ and group Ⅲ (both P<0.001). Conclusion: TPOAb is frequently present in ACA recurrent aborters and is often associated with either reduced fecundity or with poor early pregnancy outcome. Thyroid antibodies should be detected in patients with RSA.

目的探讨骨创伤后深静脉血栓（DVT）的形成与抗心磷脂抗体（ACA）以及C-反应蛋白（CRP）的关系。方法选择创伤骨折患者196例，经彩色多普勒确诊的DVT患者23例。健康查体者116例作为对照组。ELISA法检测抗心磷脂抗体，速率散射比浊法检测C-反应蛋白。结果创伤骨折患者CRP含量显著增高，但低于深静脉血栓患者；深静脉血栓患者ACA阳性率明显增高，5例ACA阳性的骨创伤患者均发生DVT；下肢骨折组、多发骨折组、骨盆骨折组CRP阳性率又高于上肢骨折组（P〈0．05）。结论ACA和CRP与骨创伤后DVT的发生相关，ACA是发生DVT的独立危险因素。

Objective To investigate the relationship among anticardiolipin antibodies (ACA),C-reactive protein (CRP) and deep vein thrombosis after skeletal trauma.Methods One hundred and ninety-six patients with skeletal trauma were chosen for the study,23 cases of which were definitely diagnosed by color Doppler.One hundred and sixteen healthy subjects were chose as control group.The level of anticardiolipin antibodies was assayed by enzyme linked immunoabsorbent assay (ELISA),and CRP was determined by rate nephelometry.Results CRP levels in patients with skeletal trauma were significantly high,but lower than in patients with deep venous thrombosis ; Positive rate of ACA in patients with deep vein thrombosis was significantly higher,five cases of ACA-positive skeletal trauma patients suffered from DVT; Positive rate of CRP in lower extremity fractures,multiple fractures group or pelvic fracture group was significantly higher than that in the upper limb fracture group (P ＜ 0.05).Conclusions ACA

目的 探讨大脑前动脉A1段(ACA-A1)变异者Willis环形态,以及与其他血管发育状况的关系.方法 回顾性分析2012年2月至8月进行健康体检的2 246名受试者的影像资料.所有受试者均无体格检查及生化检查异常,脑MR检查脑实质结构正常,且MRA排除动脉硬化引起的动脉狭窄.根据大脑前动脉A1段(ACA-A1)变异情况,将受试者分为变异组和对照组.利用x2检验比较变异组ACA-A1左右侧发育变异情况,比较变异组与对照组胚胎型大脑后动脉(FTP)的形成比例以及大脑后动脉P1段(PCA-P1)的发育情况;利用趋势x2检验比较变异组内不同ACA-A1发育变异程度与同侧FTP形成及PCA-P1发育变异情况的关系.结果 2 246名受试者中ACA-A1变异634例,正常1 612名.ACA-A1变异组中右侧429例、左侧205例,右侧变异多于左侧(x2=26.19,P＜0.01).变异组与对照组形成FTP的比例分别为22.56％(143/634)和18.30％(295/1612),变异组形成FTP比例较对照组高(x2=14.16,P＜0.01).变异组与对照组合并PCA-P1段发育不良或缺失变异比列分别为17.35％(110/634)、14.21％ (229/1612),变异组较对照组更易发生PCA-P1发育不良或缺失(x2=4.32,P＜0.05).变异组内ACA-A1发育正常侧、轻度变异侧、发育不良侧、缺失侧形成FTP的比例分别为9.15％(58/63

Objective To investigate the variations of multi-segments of the Willis'' circle in cases with ACA-A1 variation.Methods Retrospective comparative analysis was applied to images of 2 246 healthy cases which were excluded from abnomalities of physical and biochemistry examination and angiostenosis caused by angiosclerosis.We divided variant group and contrast group according to variation of ACA-A1 segment.Several aspects were carried to comparison:the variation between bilateral ACA-A1 segment within the variant group was studied by x2 test; the proportion of FTP and developing of PCA-P1 were put into test.Finally we use trend x2 test to compare the variation degree of ACA-A1,ipsilateral FTP formation and PCA-P1 variation.Results Variants of ACA-A1 contains 634 cases whereas normal cases were 1 612.A prominence in variation revealed in right side of ACA-A1 (x2=26.19,P＜0.01) which revealed by 429 cases in right side versus 205 cases in lefi side respectively.Parallelled with co