观察膀胱过度活动症(OAB)患者及健康人膀胱俞穴、下合穴与肾俞及外关穴的红外辐射温度特征,为经穴脏腑相关及穴位特异性提供科学依据。方法:以88例健康志愿者为对照,比较86例OAB患者的膀胱俞、肾俞、委中穴、外关穴的红外辐射温度,分析健康人和膀胱过度活动状态下各穴位的差异性;采用自身对照的研究方法,比较分析膀胱俞与肾俞红外辐射穴位差异性。结果:健康志愿者组与OAB组观察穴位左右两侧红外辐射温度均无明显差异(P0.05);OAB组膀胱俞的红外辐射温度明显低于健康志愿者(P0.05);两组间外关穴、肾俞、委中穴红外辐射温度比较无差异(P0.05);健康志愿者组中膀胱俞与肾俞的红外辐射温度无明显差异(P0.05);OAB组中膀胱俞的红外辐射温度较肾俞低(P0.05)。结论:在红外辐射温度方面,膀胱的腧穴与膀胱经气相关特异性优于膀胱下合穴、膀胱表里脏腑的背俞穴及与膀胱非直接相关穴位。

Objective:Compare the infrared radiation temperature of bladder Shu point , Xiahe point , BL23 , and SJ5 among Overactive Bladder ( OAB ) patients with those of healthy people , and evaluate the differences between these in two groups .The evaluations of the differences between various points provided a scientific evi-dence for organs-meridian theory and acupoint specificity .Methods:Collect infrared radiation temperature of acupoints ( BL28 , BL23 , BL40 and SJ5 ) in 88 cases of OAB patients and 86 cases of healthy people .Compare the infrared radiation temperature of BL28, BL23, BL40 and SJ5 in OAB patients with those of healthy people , and evaluate the acupoints specificity in different physical states .Through the self-control study method , ana-lyze the differences among the infrared radiation temperature of BL 28 and BL23 in OAB patients.Results:In two groups, the infrared radiation temperatures of BL23, BL28, BL40 and SJ5 in two lateral were similar (P>0.05 );the infrared radia

运用数据挖掘技术分析古代针灸治疗腰痛的使用特点。收集秦汉至清末的有关文献,进行筛选、整理并建立数据库,采用关联规则分析。共纳入针灸处方474条,涉及书籍72部,使用经穴103个,奇穴12个以及阿是穴,总频次1460次,前10位的腧穴为委中、肾俞、昆仑、申脉、足三里、涌泉、大肠俞、太冲、地机、下髎、次髎;前5位的腧穴配伍为委中→肾俞,委中→昆仑,委中→腰俞,肾俞→腰俞,委中→水沟;特定穴占总腧穴使用频次的70.14%,其中五输穴使用频次最高;腧穴主要遍布在足太阳膀胱经和足阳明胃经上,阳经使用率73.63%,下肢腧穴占总腧穴使用频次的56.23%。刺灸法中针刺占46.72%,艾灸占28.96%,针刺结合艾灸占20.02%,放血占4.31%。表明选穴方法以循经取穴为主,结合局部取穴的特点,首选阳经腧穴,腧穴主要分布在下肢,足太阳经腧穴配伍最为常用,特定穴为选穴的重要组成部分,特别重视五输穴和下合穴。刺灸法以针刺为主,放血以委中为主。

Study analytical characteristics of ancient acupuncture curing osphyalgia by using data mining tech -niques ,collecting the relevant literature from Qin Han to Qing Dynasty , screening , sorting and buliding databas-es, using association rules to analyze .474 acupuncture prescriptions are included , involving 72 books, using 103 meridian,12 extra points and Ashi acupoints , amounting 1460 using frequencies.The former 10 acupoints are Weizhong(BL 40),Shenshu(BL23),Kunlun(BL60),Shenmai(BL62),Zusanli(ST36),Yongquan(KI1), Dachangshu(BL25),Taichong(LR3),Diji(asdfa),Xialiao(BL34),Ciliao(BL32); The former 5 compatible acupoints are Wei zhong(BL40)-Shenshu(BL23),Weizhong(BL40)-Kunlun(BL60),Weizhong(BL40) -Yaoshu(DU2),Shenshu(BL23)-Yaoshu(DU2),Weizhong(BL40) -Shuigou(DU26).The specific points in the total acupoints frequencies occupying 70 .14%, of which Wushu points are frequently used;acupoints main-ly scattered in the bladder meridian and stomach meridian , the Yang meridians utilization occ

目的：初步研究电针合谷穴对痛经大鼠的镇痛效应及机制。方法：将30只处于动情间期的SD雌性大鼠随机分为3组：正常组、模型组、电针合谷组。采用苯甲酸雌二醇和缩宫素联合制备痛经大鼠模型,观察电针合谷穴对痛经大鼠扭体行为、前列腺素F2α（PGF2α）、前列腺素E2（PGE2）、β-内啡肽（β-EP）含量的影响。结果：与模型组比较,电针合谷穴组大鼠扭体潜伏期明显延长,扭体次数、总分均显著性减少（均P〈0.01）;子宫PGE2含量无显著性差异（P〉0.05）,PGF2α含量及PGF2α/PGE2比值均显著性下降（均P〈0.01）;血浆、子宫、下丘脑β-EP含量均无显著性差异（均P〉0.05）,垂体β-EP显著性升高（P〈0.01）。结论：电针合谷穴可以通过降低大鼠子宫PGF2α含量和PGF2α/PGE2比值、升高垂体β-EP含量达到止痛的作用,其镇痛效应机制可能是通过提高中枢β-EP的含量达到的。

Objective:To preliminarily study the analgesic effects of electroacupuncture ( EA ) at Hegu (LI4)point in dysmenorrhea rats and explore the mechanism .Methods:30 SD female rats aged three-month-old in the estrum were randomly divided into normal group, model group and EA group. Rats were given estradiol benzoate combined with oxytocin to prepare the models of dysmenorrhea and normal group was given the same dose of saline , EA group was given EA .The effect of EA at Hegu on the writhing behavior , PGE2、PGF2α、β-EP levels in dysmenorrheal rats were observed .Results:To compared with model group , the rats''writhing incuba-tion period in EA at LI4 group was obviously prolonged ,rats''writhing number and scores were decreased signifi-cantly(all P 0. 05);PGF2αlevel and PGF2α/PGE2 ratio were reduced significantly in EA group (all P 0.05), in pituitary that was significantly increased(P <0.01).Conclusions:Electro-acupuncture at Hegu point can reduce PGF 2αlevels and PGF2α/PG

目的：观察齐刺胆的下合穴对实验性胆绞痛豚鼠血浆β-内啡肽（β-EP ）和八肽胆囊收缩素（ CCK-8）的影响以及镇痛效果，为临床治疗胆绞痛选择新的方法提供科学依据。方法：将60只成年雄性豚鼠随机分为正常组（10只）、模型组（10只）、药物组（20只）和齐刺组（20只）。正常组不作任何处置正常饲养，模型组、药物组、齐刺组均造模。造模成功后，药物组予双氯芬酸钠盐酸利多卡因注射液腹腔注射；齐刺组取双侧阳陵泉穴进行齐刺，每日1次，连续治疗5天。治疗结束后15 min内处死各组豚鼠，腹主动脉采取新鲜血液，匀浆、离心后分离血浆，采用放射免疫法测定β-EP和CCK-8的含量。结果：模型组与正常组比较，血浆中β-EP和CCK-8含量显著升高（ P＜0．01）；药物组和齐刺组血浆中β-EP和CCK-8含量均比模型组明显降低，有统计学意义（P＜0．01）；药物组、齐刺组比较无明显差异，无统计学意义（ P＞0．05）。结论：齐刺法刺激阳陵泉穴对实验性胆绞痛豚鼠有镇痛作用，可能与改善豚鼠血浆β-EP和CCK-8的表达水平有关。

Objective:To observe the effect of analgesia as well as the influence on endorphin (β-EP) and cholecystokinin-octopeptide ( CCK-8) in the plasma of the experimental biliary colic guinea, which provides a scientific basis for choosing a new way to treat biliary colic.Methods:60 adult male guinea pigs were divided into 4 groups: the normal group ( 10 guinea pigs ) , the model group ( 10 guinea pigs ) , the drug group ( 20 guinea pigs) and the triple needling group (20 guinea pigs).The normal group was fed normally without any processing,but other groups made models.In the drug group, give a intraperitoneal injection with compound di-clofenac sodium and lidocaine hydrochloride injection after making models successfully; in the triple needling group, triple needled bilateral GB34 and needled once a day continuously treating for 5 days.At the end of the treatment, sacrifice the guinea pigs in each group, and fresh blood,homogenate were taken for the examination of theβ-EP and CC

目的 评价电针对内毒素性休克兔急性肾损伤的影响及其与Kelch样环氧氯丙烷相关蛋白-1(Keap1)-转录因子NF-E2相关因子2(Nrf2)/抗氧化反应元件(ARE)信号通路的关系.方法 清洁级健康成年雄性新西兰大白兔40只,体重1.5 ～ 2.0 kg,采用随机数字表法,将其分为4组(n=10):对照组(C组)、内毒素性休克诱发急性肾损伤组(AKI组)、电针+内毒素性休克诱发急性肾损伤组(EA组)、非穴位电针+内毒素性休克诱发急性肾损伤组(SEA组).采用耳缘静脉注射脂多糖(LPS)5 mg/kg(溶于2 ml生理盐水)建立兔内毒素性休克诱发急性肾损伤模型.EA组电针足三里穴和肾俞穴,1次/d,每次持续10 min,连续4d,刺激参数:疏密波,频率2/15 Hz,波宽0.2 ～ 0.6 ms,电流1～2mA,以兔出现轻微肌颤为度,留针1h出针,第5天建立模型,持续电针刺激至处死.SEA组选择穴位旁开0.5 cm部位行电针刺激,其余与EA组相同.于注射LPS后6h时,取尿液2ml,测定尿α1微球蛋白(α1-MG)浓度.HE染色下观察肾脏病理学结果,并行肾脏组织学(HSK)评分.干湿重法测定肾组织含水率,RT-PCR法测定Nrf2 mRNA、血红素氧合酶-1(HO-1) mRNA,Western blot法测定肾组织Nrf2总蛋白、核蛋白及下游HO-1蛋白表达水平.结果 与C组比较,AKI组、EA组、SEA组HSK评分、肾组

Objective To evaluate the effects of electro-acupuncture (EA) at Zusanli and Shenshu acupoints on endotoxic shock-induced acute kidney injury (AKI) and the relationship with Keap1-Nrf2/ARE signaling pathway in rabbits.Methods Forty pathogen-free male New Zealand white rabbits,weighing 1.5-2.0 kg,were randomly divided into 4 groups randomly (n =10 each) using a random number table:control group (group C) ; endotoxic shock-induced AKI (group AKI) ; EA + endotoxin-induced AKI group (group EA) ; EA at non-acupoints + endotoxin-induced AKI group (group SEA).Endotoxic shock-induced AKI was induced with LPS 5 mg/kg (in 2 ml of normal saline) injected via the auricular vein.Electro-stimulation (2/15 Hz,0.2-0.6 ms,1-2 mA) of Zusanli and Shenshu acupoints was performed for 10 min once a day for 4 consecutive days,and the model was established on 5th day and then EA was continued until the animals were sacrificed in group EA.In group SEA,EA was performed at the points 5 mm lateral to th